STAGE TOP CHAMBER
The Stage Top Chambers fits in the XY stage or PIezo insert of the microscope, connects to Okolab Temperature, Gas and Humidity Controllers and creates the proper environment for live cell imaging right on the microscope stage.
- Temperature Range: from 3°C above ambient to 60°C
- Temperature Accuracy: ± 0.1°C in sample feedback mode, ± 0.3°C in chamber feedback mode
- Compatible with: Bold Line T, UNO Combined and H401-T Controllers
- Chambers available for any XY stage / piezo insert
- Embedded temperature sensor in heated glass lid and in chamber body
- Interchangeable magnetic inserts allow to host Petri, Slides and MW Plates
- Magnetic locks hold the Petri, the Slides and the MW Plates in the correct position inside the chamber
- Perfusion holes available for inlet and outlet of tubes
- Sliding lid allows easy access to the sample
- Optional lids are available for Koehler Illumination, laser safety and injection during imaging
Need a SLIM profile ?
Remove the chamber riser and use the Koehler Lid.
Perfusion ?
Each chamber features up to 12 holes for inlet and outler of perfusion tubings
Different sample holder ?
Change the chamber insert. It is a 5 seconds task with the new magnetic sample holders
Keep the sample in the correct position by using the Magnetic Locks
Chambers available for any XY stage / piezo insert
Manufacturer | Model or stage opening | Climate Chamber | Fixing tool | ||||||||||||||||||||
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Applied Precision | 51-603288-000 Deltavision Stage | H301-K-FRAME | DV-PA | ||||||||||||||||||||
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Mad City Labs | Z100/Z500 Piezo stages | H301-MCL-Z100/500 | - | ||||||||||||||||||||
Marzhauser | Scan IM series (110x160mm opening) | H301-K-FRAME | - | ||||||||||||||||||||
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Thorlabs | Thorlabs MLS203-1 /MLS203-2 | H301-K-FRAME | THORPA | ||||||||||||||||||||
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Upright Microscopes | any stage for uprightmicroscopes | H301-UP | - |
TEMPERATURE CONTROLLER
H301-T-UNIT-BL-PLUS is the most advanced solution for Temperature Control. It is compatible with Digital Gas Controllers and with Active Humidity Controller to ensure best environmental control for live cell imaging.
H301-T-UNIT-BL-PLUS has five independent temperature channels: chamber base, chamber lid, objective heater, sample temperature sensor, room temperature sensor.
- Temperature Range: from 3°C above ambient to 60°C
- Temperature Resolution: 0.1°C
- Temperature Accuracy: ± 0.1°C in sample feedback mode, ± 0.3°C in chamber feedback mode
- Controls up to # 5 Temperature sensors:
- Chamber Base
- Chamber Lid
- Sample T Sensor
- Room T Sensor
- Objective Heater T sensor
- Compatible with:
- Objective Heater
- Active Humidity Controller
- Digital Gas Controllers
- Smart Box data logger
- SDK available for integration in third party software
- Drivers available for: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager
- TTL signal option order: H301-T-UNIT-BL-PLUS-TTL
Touch Pad Interface
Beautifully designed, simple to use and very compact.
OKO-TOUCH is all you need to operate the system.
Compatible Units
H301-T-UNIT-BL-PLUS is designed to perfectly fit with:
- Active Humidity Controller
- CO2-O2-UNIT-BL
- Smart Box data logger
- OKO-TOUCH
Sample Temperature Sensor?
It measures the temperature in a reference well near the sample.
It ensures Temperature stability even if the doors of the enclosure are temporarely opened.
CO2 - O2 CONTROLLERS
The Bold Line Gas Controllers connect to the Temperature Controller via a cable-less connector and are operated through the same touch screen interface OKO-TOUCH.
CO2 and or O2 accuracy is ±0.1% with adjustable total output flow rate.
The Active Humidity Controller is typically used with a Stage Top Incubation System.
Features
- CO2 accuracy: 0.1% (see Controller page for more info)
- O2 accuracy: 0.1% (see Controller page for more info)
- Touch Screen Interface - OKO-TOUCH
- Output Flow Rate: adjustable from OKO-TOUCH
- Compatible with Active Humidity Controller
- Programmable CO2 (and /or) O2 cycles
- Compatible with SMART BOX for data logging and remote operation - Optional
- Data Logging via OKOLAB Data Log Software - Optional
- Compatible with: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager
- TTL signal compatibility, upon request
Preventing evporation?
Use the best Humidifier on the market: ACTIVE HUMIDITY CONTROLLER
It connects to the Gas Controller and shows up in OKO-TOUCH
Hypoxia and / or Hyperoxia ?
Oxygen control is our pride.
Check out the available solutions in the Controller List
Multiple units ? Be Smart - go central
One controller feeds up to 10 microscope incubators.
Microscopes can be in different rooms.
Available Bold Line Digital Controllers
CO2 | O2 | Accuracy | Outlet P, barg (psi) | |
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CO2-Unit-BL | 0-18% | - | ±0.1% | 0 |
CO2-Unit-BL-CP2 (multi-user centralized controller) | 0-10% | - | ±0.1% | 1.5 (22) |
O2-Unit-BL [1-20] | - | 1-20% | ±0.1% | 0 |
CO2-O2 Unit-BL [0-10;1-18] | 0-10% | 0-20% | ±0.1% | 0 |
CO2-O2 Unit-BL [0-20;1-95] | 0-20% | 1-95% | ±0.1% | 0 |
CO2-O2 Unit-BL [0-10; 0-1] | 0-10% | 0-1% | ±0.1% | 0 |
ACTIVE HUMIDITY CONTROLLERS
Active Humidity Controllers control the Relative Humidity inside the chamber in the range 50-95% with a resolution of 1%.
Features
- Connects to any Bold Line Gas controller
- Controlled via OKO-TOUCH
- Humidity sensor resolution 1%
- A heated tube connects the Active Humidity Controller to the Chamber
- Stand alone version (HM-ACTIVE-STANDALONE) is available to run the humidifier independently of the gas controller.
The dry gas is humidified by flowing over warm water. The Humidity sensor regulates water temperature in order to achieve the desired relative humidity in the chamber.
To prevent water condensation, the tube connecting the humidity controller to the chamber is heated.
OKO-TOUCH
OKO-TOUCH is an intuitive and user-friendly touch screen interface. It connects to any one of the Bold Line Controllers, detects all equipment in the system and displays all functions supported by the equipment detected.
OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition data logging is possible via SMART-BOX (that also allows remote control), via DATA-LOG Okolab software (that also allows to set enhanced thermal/gas cycles), and via following third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN Black software is available upon request.
It allows to:
- Turn all Bold Line Controllers on/off
- Define set point values
- Set flow rates gas controller
- Define gas cycles of gas controller
- Define temperature cycles of temperature controller
- Select sample or chamber feedback mode
- Select chamber model and plate adapter
- Display all set points and measured parameters
- Run self calibration procedures