You should use an objective heater, if you:
Because, if you don't, sample temperature will drop due to oil contact with the cold objective, as shown in the following graph.
Step 1: Objective not in contact with Petri 2; Objective Heater Off.
Step 2: Objective in oil contact with Petri 2; Objective Heater Off.
Step 3: Objective in oil contact with Petri 2; Objective Heater on, Offset value 5.3°C.
During the self calibration routine, the offset value of the objective heater is progressively increased, until T2 reaches the desired set point temperature of 37.0°C.
Figure 3 reports the profile of T1, T2, and of Objective Heater Temperature during the experimental campaign described above.
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